Laser light scattering as a probe of sperm motility


M.W. Woolford, J.K. Woolhouse, D.S.M. Phillips, S.A. Hawley; J.D. Harvey and P. Shannon, B. Curson

Ruakura Animal Research Station, Hamilton; University of Auckland and New Zealand Dairy Board, Newstead, Hamilton

Proceedings of the New Zealand Society of Animal Production. 1980, 40: 124-129

Practical aspects of assaying live and dead fractions of bull spermatozoa, as well as concentration effects and organism survival, are briefly discussed. The technique and physical basis for the use of laser light scattering in the assay is presented. Previous work assumes that the high frequency signal components arise from translation motion. Our recent work demonstrates that sperm rotation is responsible for the high frequency photomultiplier-detector signals.

Keywords: NZSAPAB;


Last Updated 12-09-1998