The development and application of ELISA detection of
Lolium endophyte in ryegrass staggers research
D.R. Musgrave and L.R. Fletcher
Plant Diseases Division, Department of Scientific and Industrial
Research, Lincoln and Grasslands Division, Department of Scientific and
Industrial Research, Lincoln
Proceedings of the New Zealand Society of Animal Production.
1984, 44: 185-188
An enzyme-linked immunosorbent assay (ELISA) system has been
developed for the detection of an endophytic fungus of Lolium
perenne. The technique has been used to demonstrate quantitative
differences in the spatial and tissue distribution of the endophyte in
vegetative and reproductive tillers of infected plants. The influence
of this on pasture toxicity is discussed.
The nature of the endophyte antigen is described and the relationship
between the antigen and toxicity discussed.
Keywords: NZSAPAB;
ELISA; Lolium endophyte; Lolium perenne L.
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Last Updated 03-05-1997
