Four sheep were catheterised in branches of the deep circumflex iliac artery and vein to allow measurement of arterio-venous concentration differences and blood flow (using dye dilution techniques) in a 560 cm¾ patch of skin on the lateral abdominal flank.
Blood flow to the infused area ranged between 5 and 30 ml/min, giving an average for the total skin of about 7% of cardiac output. Net uptake of amino acids (tyrosine and phenylalanine) across the area gave a net protein gain by the total skin of 3 to 4 g/d. Uptake of 14C-tyrosine across the skin patch gave an estimate of protein synthesis of 10 g/d in the total skin.
It is intended to develop the preparation to allow comparisons of protein:energy metabolism in both normal and fleece weight selected sheep.
Keywords: NZSAPAB; Skin; blood flow; protein synthesis; sheep