Measurement of blood flow and amino acid metabolism in the skin of sheep


P.M. Harris, D.W. Dellow and B.R. Sinclair

Biotechnology Division, DSIR, Palmerston North

Proceedings of the New Zealand Society of Animal Production. 1988, 48: 187-190

This paper reports preliminary studies to determine the normal variation in blood flow, amino acid uptake and protein metabolism in a defined area of skin on 45 kg Romney wethers.

Four sheep were catheterised in branches of the deep circumflex iliac artery and vein to allow measurement of arterio-venous concentration differences and blood flow (using dye dilution techniques) in a 560 cm¾ patch of skin on the lateral abdominal flank.

Blood flow to the infused area ranged between 5 and 30 ml/min, giving an average for the total skin of about 7% of cardiac output. Net uptake of amino acids (tyrosine and phenylalanine) across the area gave a net protein gain by the total skin of 3 to 4 g/d. Uptake of 14C-tyrosine across the skin patch gave an estimate of protein synthesis of 10 g/d in the total skin.

It is intended to develop the preparation to allow comparisons of protein:energy metabolism in both normal and fleece weight selected sheep.

Keywords: NZSAPAB; Skin; blood flow; protein synthesis; sheep


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Last Updated 18-03-1997