Comparison of commercial gonadotrophins using bioassays
K.M. Henderson, A. Weaver, R.L. Wards, S. Lun, K.P. McNatty
Wallaceville Animal Research Centre, MAF Technology, P.O. Box
40063, Upper Hutt, New Zealand
Proceedings of the New Zealand Society of Animal Production.
1990, 50: 161-166
The FSH:LH bioactivity ratios, determined by radioreceptor
assays, of four commercial gonadotrophin preparations were Folligon 5;
F.S.H.-P. 18; Folltropin 49 and Ovagen 1090. The mean number of oocytes
produced in immature rats after ovulation induction following
administration of these gonadotrophin preparations by injection
(Folligon, 10 to 50 i.u.) or 48h continuous infusion (others, 30 to 1000
ug/day) was dose-dependent, except at the highest doses when mean oocyte
numbers either remained unchanged, or fell significantly in the cases of
Folligon and F.S.H.-P. The highest mean number of oocytes produced in
response to Folltropin (48+9) and Ovagen (47+7) were significantly
higher (P<0.05) than those attained with Folligon (21+6) or F.S.H.-P
(31+5). Mean ovarian weights also increased in a dose-dependent fashion
in response to each of the gonadotrophin preparations. Co-infusion of
NIADDK-ovine LH-25 at 10 to 20 ug/day with Ovagen (250 ug/day) or
NIADDK-ovine FSH-17 (10 ug/day) (both low in LH activity) increased mean
oocyte production 1.5 to 3-fold. Co-infusion of ovine LH at 40 ug/day
significantly reduced mean oocyte numbers.
Keywords: NZSAPAB;
Bioassay; rats; gonadotrophin; FSH; LH; oocytes; superovulation
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Last Updated 25-01-1997
