The use of unfrozen semen for in vitro fertilisation of
in vitro matured bovine oocytes
A.D. Seaton, J.W. Catt, S.L. Rhodes, M.F. McDonald, R.A.S. Welch
Grasslands Division, DSIR, Private Bag, Palmerston North, New
Zealand
Proceedings of the New Zealand Society of Animal Production.
1991, 51: 67-72
The use of unfrozen, diluted bovine semen for in vitro
fertilisation (IVF) was investigated. In vitro matured bovine
oocytes were incubated with various (0.125 - 4.0 x 10 6/ml)
concentrations of washed sperm. Penetration rates ranged from 21% at
low sperm concentrations to 80-100% above 1 x 10 6 sperm/ml (P<0.01).
Unfrozen sperm gave better penetration rates than frozen-thawed sperm.
Oocytes were incubated with washed sperm (1 x 10 6/ml) in increasing
dilutions of caprogen extender. Sperm penetration was significantly
inhibited at dilutions of 10-fold or less (P<0.01). Oocytes were
inseminated with washed sperm (1 x 10 6/ml) at various concentrations
(0.50 ug/ml) of heparin. No significant differences were found.
Keywords: NZSAPAB;
Bovine; IVF; sperm; extender; heparin
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Last Updated 25-01-1997
