Antibody-mediated enhancement of growth hormone activity:
application to animal production
R. Aston, A.T. Holder, D.A. Rathjen, T.E. Trigg, B.A. Moss, J.A.
Pell
Peptide Technology Ltd, P.O. Box 444, Dee Why, NSW 2099,
Australia
Proceedings of the New Zealand Society of Animal Production.
1991, 51: 227-234
The binding of monoclonal antibodies (MAbs) of predetermined
specificity to growth hormone (GH) can result in significant enhancement
of the biological activity of the hormone in vivo. The
topographic region on GH associated with the enhancement phenomenon has
been localised to two proximate sites on adjacent loop regions by
extensive peptide synthesis (sequences: 35-53 and 134-154). The
application of these observations of GH and (ii) increased production
parameters following administration of particular antibodies. The
latter aspect has now been demonstrated in a number of animal models by
the passive vaccination of animals with purified anti-peptide antisera
or monoclonal antibodies. Production parameters affected in such
experiments include growth rate, fat metabolism, diabetogenic activity
and lactation in ewes. At the present time, it has been shown that
peptide sequence 134-154 will elicit up to 80% high titre, immuno-
positive responders in sheep. Monitoring the growth rate and other
production parameters of lambs vaccinated with sequence 134-154
indicates improved growth and total protein characteristics. These
observations, in conjunction with the development of methodologies for
effective autoimmunization of animals, may provide a basis for the
design of peptide vaccines to improve animals, may provide a basis for
the design of peptide vaccines to improve animal production.
Keywords: NZSAPAB;
Growth hormone; immunisation; antibodies
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Last Updated 25-01-1997
