The use of protein polymorphism and DNA fingerprinting to solve
complex pedigree problems in deer - Brief Communication
M.L. Tate, K.G. Dodds, K.M. McEwan, F.C. Buchanan, P.A.
Swarbrick
AgResearch, Invermay Research Centre, P.O. Box 50034, Mosgiel, New
Zealand
Proceedings of the New Zealand Society of Animal Production.
1992, 52: 143-144
We examined the practicality of using a combination of protein
polymorphism and DNA fingerprinting to match up hinds and calves in nine
separate mating groups of red deer with between 8 and 21 progeny each.
Variation in 10 polymorphic proteins excluded 0.61 to 0.89 of the
possible dam-calf combinations in each sire group, leaving between 2 and
200 pedigree combinations per group to be solved by DNA fingerprinting.
Of the 44 sire-dam-calf combinations tested by DNA fingerprinting, 22
were excluded, while four calves matched the parents at all bands.
However, 16 pedigrees were equivocal, due to the fact that one or both
parents were not in a lane directly adjacent to the calf on the
fingerprinting gel. The low probability of exclusion of protein testing
in some sire groups, combined with the practical limitation of having to
run each possible pedigree combination side by side on DNA
fingerprinting gels, mean that the present techniques do not provide a
routine method for identifying the dam-calf pairings in red deer mating
groups on farms.
Keywords: NZSAPAB;
Deer; pedigree; parentage; protein polymorphism; DNA
fingerprinting
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Last Updated 25-01-1997
